Small retrospective cohorts using the MDRD equation have reported an underestimate of true GFR in diabetes and large prospective studies are urgently required.
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In an example reaction, liug of a nucleic acid molecule is incubated at 37 C for 1 hour with 1-10 units of T4 RNA ligase (New England Biolabs, Ipswich, MA) according to the manufacturer's protocol
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